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KMID : 1234420090370040361
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 4 p.361 ~ p.364
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Trehalose Protects Activity of Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000.
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Kang Min-Jung
Lee Jong-Hwan
Lee Bok-Kyu
Kim Kwang-Hyeon
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Abstract
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Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS tends to be decreased by heat. To investigate whether trehalose can protect AYS activity against heat, comparative studies on the AYS with and without trehalose were performed. After heat treatment at high temperatures (50oC-70oC), the AYS with trehalose had higher activity than the AYS without trehalose had. In case of AYS with trehalose (0.3-0.9M), activity of the AYS could be determined at ranging from 7.8 μg/mL to 31.3 μg/mL on S. cerevisiae by MTT method. Consequently, activity of the AYS after heating was well maintained by trehalose.
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KEYWORD
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Anti-yeast substance, Rahnella aquatilis, trehalose, heat protection., Saccharomyces cerevisiae
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